Embedded Files
Live-cell Airyscan image of a primary mouse hepatocyte. TMRE labels mitochondria; PicoGreen labels nuclei. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
Live-cell Airyscan image of a primary mouse hepatocyte. TMRE labels mitochondria; PicoGreen labels nuclei. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
Live-cell SIM image of HeLa cell mitochondria stained with MitoTracker, highlighting cristae. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
Live-cell SIM image of HeLa cell mitochondria stained with MitoTracker, highlighting cristae. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
Live-cell Airyscan image of cultured pancreatic beta cell (INS-1). TMRE labels mitochondria; PicoGreen labels nucleus and mtDNA. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
Live-cell Airyscan image of cultured pancreatic beta cell (INS-1). TMRE labels mitochondria; PicoGreen labels nucleus and mtDNA. Image acquired by Dane M. Wolf, PhD (Shirihai Lab, UCLA).
How it Works:
Step 1. Acquire high-resolution images of mitochondria
Fluorescent dyes or immunocytochemistry reveal mitochondrial networks and other subcellular structures.
Step 2. Split multichannel images into individual fluorescence channels
Separate markers for mitochondria, nuclei, or mtDNA allow precise downstream quantification.
Step 3. Segment individual organelles from background
Using advanced segmentation pipelines, mitochondria are accurately identified as distinct objects.
Step 4. Quantify mitochondrial morphology and features
Generate readouts such as aspect ratio, circularity, count, area, colocalization, and more. Data are visualized and statistically analyzed for biological interpretation.
Need help analyzing your mitochondrial images?
Need help analyzing your mitochondrial images?
Whether you're working with live-cell microscopy, fixed samples, or multi-channel datasets, MitoGraphica streamlines the path from raw images to biological insight.
We offer:
Expert consultation on mitochondrial structure and function
Custom image analysis tailored to your experimental system
Quantitative outputs including morphology, membrane potential, colocalization, and more
Publication-ready figures and statistical reports
📩 Get in touch to discuss your project → contact@mitographica.com
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